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Modulation of marrow stromal cell function using poly(D,L-lactic acid)-block-poly(ethylene glycol)-monomethyl ether
A Göpferich1, S J Peter, A Lucke
1Department of Pharmaceutical Technology, University of Regensburg, 93040 Regensburg, Germany. achim.goepferich@chemie.uni-regensburg.de
Journal of Biomedical Materials Research
|July 9, 1999
Summary
Poly(D,L-lactic acid) polymers supported osteoblast adhesion and proliferation. Poly(ethylene glycol)-poly(D,L-lactic acid) block copolymers regulated protein adsorption and cell differentiation, suggesting tunable biomaterial properties.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Cell Biology
Background:
- Poly(D,L-lactic acid) (PLA50) is a common biomaterial for tissue engineering.
- Controlling protein adsorption and cell behavior on biomaterials is crucial for effective applications.
- End-capped and non-end-capped PLA50, along with a poly(ethylene glycol)-PLA50 block copolymer, were evaluated.
Purpose of the Study:
- To investigate the influence of PLA50 molecular weight and end-capping on osteoblast adhesion and protein adsorption.
- To compare the cellular response to PLA50 polymers versus a poly(ethylene glycol)-PLA50 block copolymer.
- To determine the potential of block copolymers for regulating protein adsorption and cell behavior.
Main Methods:
- Synthesis and characterization of end-capped and non-end-capped PLA50 polymers with different molecular weights.
- Preparation of a poly(ethylene glycol)-PLA50 diblock copolymer.
- In vitro assessment of marrow stromal osteoblast adhesion and proliferation on the different polymer surfaces.
- Fetal bovine serum (FBS) protein adsorption studies using electron spectroscopy.
Main Results:
- Both end-capped and non-end-capped PLA50 polymers supported comparable cell attachment and proliferation.
- The poly(ethylene glycol)-PLA50 block copolymer did not support cell proliferation but promoted highly differentiated and metabolically active attached cells.
- FBS proteins adsorbed readily to PLA50 surfaces but showed significantly reduced adsorption to the block copolymer.
- Surface analysis confirmed differential protein adsorption based on polymer composition.
Conclusions:
- PLA50 polymers provide a suitable substrate for osteoblast adhesion and proliferation.
- Poly(ethylene glycol)-PLA50 block copolymers can modulate protein adsorption and influence cell differentiation.
- Varying block copolymer composition offers a strategy to control protein adsorption and subsequent cell adhesion for biomaterial design.
Keywords:
Non-programmatic