An Effective Fluorescent Sensor for Choline-Containing Phospholipids

A set of weak CH small middle dot small middle dot small middle dotO interactions is responsible for the recognition of tetraalkylammonium ions (ball-and-stick structure) by the tripodal receptors depicted in the diagram, in which the binding pocket is lined with squaramide units. The association constants of the fluorescent receptors are remarkably high at about 10(5) M(-1) and can be used for signaling the presence of selected choline phospholipids. Z=RNH, where R=alkyl, arylamino; X(-)=BH(4)(-), Cl(-), Br(-), I(-).