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Generation, identification, and characterization of repair-defective mutants of Arabidopsis

A Britt1, C Z Jiang

  • 1Section of Plant Biology, University of California, Davis, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 12, 1999
PubMed
Abstract

No abstract available in PubMed .

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Mismatch Repair01:36

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DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
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Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
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