Related Experiment Video
Updated: Oct 11, 2026
Neonatal Pial Surface Electroporation
Published on: May 7, 2014
[Proliferative zones of the epithelium of human embryonic brain choroid plexuses]
1Department of Morphology, Russian Academy of Medical Sciences, St. Petersburg.
Insights
Human embryo studies reveal distinct proliferative zones in the choroid plexus. Differentiating cells show fewer nucleoli than proliferating cells, aiding in understanding early brain development.
Area of Science:
- Developmental biology
- Neuroscience
- Human embryology
Context:
- Investigating early human brain development.
- Focusing on the choroid plexus in 7-13 week old embryos.
- Utilizing immunocytochemical detection of PCNA.
Purpose:
- To identify the proliferative zones within the telencephalic and rhombencephalic choroid plexus.
- To correlate nucleoli number with cell proliferation and differentiation stages.
Summary:
- Proliferative zones in the telencephalic choroid plexus are located at the base, bordering the ventricular zone.
- In the rhombencephalic choroid plexus, proliferation occurs at the angle formed by the tectum and the IV ventricle floor.
- Epithelial cells undergoing differentiation exhibit fewer nucleoli compared to actively proliferating cells.
Impact:
- Provides precise localization of proliferative regions in the developing human choroid plexus.
- Offers a cellular marker (nucleoli number) for distinguishing proliferating from differentiating cells.
- Contributes to a deeper understanding of early neurodevelopmental processes and potential origins of congenital brain abnormalities.
Abstract:
The study performed in 7-13 wks old human embryos by using immunocytochemical detection of PCNA, the proliferation marker, has demonstrated that in choroid plexus of telencephalon proliferative zone is located in the area of the base of the plexus--at the border with the ventricular zone of brain cortex anlage and in choroid plexus of rhombencephalon--in the zone of the angle formed by the tectum in choroid plexus and fundus of the IV ventricle. Comparison with the immunocytochemical study data with the mean number of nucleoli in epitheliocyte nuclei indicates that cells of choroid plexus epithelium leaving stage G of mitotic cycle and starting to differentiate have lower number of nucleoli than proliferating cells.
