The carbon paste electrode encrusted with a microreactor as glucose biosensor

Area of Science:

• Electrochemistry
• Biosensors
• Analytical Chemistry

Background:

• Amperometric biosensors are crucial for glucose monitoring.
• Carbon paste electrodes (CPEs) offer a versatile platform for biosensor development.
• Microreactors (MRs) can enhance biosensor performance and stability.

Purpose of the Study:

• To construct and characterize novel amperometric biosensors for glucose determination.
• To investigate the role of microreactors in enhancing biosensor stability and sensitivity.
• To optimize biosensor components including glucose oxidase (GO), mediator (M), and acceptor (A).

Main Methods:

• Fabrication of microreactors (MRs) using CPC-silica carrier (CPC) loaded with GO, mediator, and acceptor.
• Construction of amperometric biosensors based on carbon paste electrodes (CPEs) encrusted with MRs.
• Electrochemical measurements at a potential of 0.15–0.27 V versus Ag-AgCl.
• Optimization of GO, mediator, and polymer amounts.
• Digital modeling of biosensor action.

Main Results:

• Linear calibration graphs for glucose were observed in the range of 1.5–50 mM.
• Biosensor sensitivity remained stable across a pH range of 6–8.
• Dissolved oxygen had a minor influence (7%) on the biosensor response.
• Ascorbic acid showed minimal interference.
• Biosensors demonstrated high stability, maintaining response for at least 6 months.
• Optimization of components improved biosensor performance.

Conclusions:

• Novel amperometric biosensors utilizing microreactors provide a stable and sensitive platform for glucose detection.
• The microreactor design enhances the long-term operational stability of the biosensors.
• The developed biosensors show potential for reliable glucose monitoring applications.