Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression profiling: DNA arrays in many guises.

S Granjeaud1, F Bertucci, B R Jordan

  • 1TAGC Group, Institut de Cancérologie et d'Immunologie de Marseille, CIML INSERM/CNRS, Marseille, France.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|August 26, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathological complete response with neoadjuvant pembrolizumab and chemotherapy in non-metastatic triple-negative inflammatory breast cancer.

ESMO open·2026
Same author

Corrigendum to "Sorafenib in patients with locally advanced and metastatic chordomas: a phase II trial of the French Sarcoma Group (GSF/GETO)": [Ann Oncol 26 (2015) 2168-2173].

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Expression of antibody-drug conjugate targets in soft tissue sarcomas.

ESMO open·2025
Same author

Twenty-year survival of advanced gastrointestinal stromal tumours treated with imatinib: exploratory long-term follow-up of the BFR14 trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Regorafenib as maintenance therapy after first-line doxorubicin-based chemotherapy in advanced non-adipocytic soft tissue sarcomas patients: a double-blind randomised trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Clinical description and development of a prognostic score for neurofibromatosis type 1 (NF1)-associated GISTs: a retrospective study from the NETSARC.

ESMO open·2025
Same journal

AI in Genomics: From Variant Calling to Multi-Omics Integration.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Rethinking One Health: Microbial Foundations for Ecological Governance.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Biobanked Liver Organoids: A Roadmap for Precision Hepatology.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

The Temporal Architecture of Human Cells: Organelle Clocks and Distributed Circadian Time.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Opposing Activity at the Apical Surface: An Antagonistic Collaboration Between Crumbs and Myosin II Determines Organ Shape.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Hidden Fungal DNA Structures May Shape Sequencing Outcomes.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
See all related articles

DNA arrays are essential for measuring gene expression, aiding in the functional characterization of newly sequenced genes. Different array methods exist, with sensitivity and accessibility being key factors in choosing the best approach, not always the most advertised ones.

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • DNA arrays are crucial for large-scale gene expression measurement.
  • Abundant sequence data necessitates expression profiling for functional characterization.
  • Expression data helps in understanding newly discovered genes from DNA sequencing.

Purpose of the Study:

  • To compare different DNA array implementations for gene expression measurement.
  • To evaluate methods based on sensitivity, sample requirements, and accessibility.
  • To challenge the notion that advertised technologies are always superior.

Main Methods:

  • Comparison of various DNA array technologies based on hybridization principles.
  • Analysis of sensitivity and sample input requirements for each method.

Related Experiment Videos

  • Assessment of accessibility for academic researchers.

    • Main Results:

    • Multiple DNA array implementations exist, all based on RNA-DNA hybridization.
    • Sensitivity, sample needs, and accessibility vary significantly among methods.
    • Widely advertised technologies like glass microarrays and oligonucleotide chips are not universally optimal.

    Conclusions:

    • The choice of DNA array method should consider practical factors beyond marketing.
    • Sensitivity and sample requirements are critical for effective gene expression analysis.
    • Accessible and sensitive methods may be preferable for academic research over high-profile but less practical options.