Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD44 Expression in Benign and Neoplastic Human Prostates.

Dhir1, Gau, Krill

  • 1Department of Pathology, University of Pittsburgh School of Medicine, Pittsburg, Pennsylvania, USA

Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
|August 27, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early voiding dysfunction associated with prostate brachytherapy.

Urologic oncology·2000
Same author

Editorial: improving prostate cancer detection

The Journal of urology·2000
Same author

Evaluation of an electronic blood pressure device for epidemiological studies.

Blood pressure monitoring·1999
Same author

Expression of the Cell Adhesion Molecule CD44 in Human Lung Tumors and Cell Lines.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·1999
Same author

Isolation and unambiguous synthesis of cryptolepinone: An oxidation artifact of cryptolepine

Journal of natural products·1998
Same author

Hydrophobic Aggregation in Polyurethane Ionomer Solutions.

Journal of colloid and interface science·1998
Same journal

Summary of recent deal activity.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2013
Same journal

Diagnostic conferences : january-june 2006.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2013
Same journal

A novel 1297-1304delGCCTGCCA mutation in the exon 10 of the thyroid hormone receptor β gene causes resistance to thyroid hormone.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2013
Same journal

Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2006
Same journal

Prevalence of four virulence genes in Campylobacter jejuni determined by PCR and sequence analysis.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2006
Same journal

Diagnostic proceeding in Silver-Russell syndrome.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2006
See all related articles

CD44 standard (CD44s) expression is increased in malignant prostate cancer compared to benign tissues. This suggests CD44s may serve as a biomarker for prostate cancer detection and progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • CD44 is a cell surface glycoprotein and hyaluronic acid receptor implicated in epithelial neoplasms.
  • Altered CD44 expression levels are associated with prognostic implications in various cancers.
  • Investigating CD44 isoforms in prostate tissue is crucial for understanding malignant transformation.

Purpose of the Study:

  • To assess the expression of standard (CD44s) and variant (CD44R, v5, v6, v7/8, v10) CD44 isoforms in normal and cancerous human prostate tissues and cell lines.
  • To evaluate the potential of CD44 expression patterns as a biomarker for prostate cancer diagnosis and progression.

Main Methods:

  • Utilized reverse transcription polymerase chain reaction (RT-PCR), Western blotting, and immunofluorescence to analyze CD44 expression.

Related Experiment Videos

  • Examined prostate tissue (benign and malignant) and various prostate cell lines (DU-145, PC-3, LNCaP, p69) and primary cultures.

    • Main Results:

    • CD44 standard (CD44s) expression was detected in both normal and neoplastic prostatic tissues.
    • Neoplastic prostate tissues showed higher CD44s expression compared to benign tissues (all neoplastic vs. 50% benign).
    • Benign prostate tissues exhibited higher CD44 variant 5 (v5) protein levels than neoplastic tissues.

    Conclusions:

    • Prostate adenocarcinoma cells, with the exception of LNCaP, express CD44s.
    • Distinct CD44 expression patterns differentiate benign from neoplastic prostate tissues.
    • CD44s expression serves as a potential biomarker for malignant transformation in the prostate.