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Related Experiment Videos

Plug flow cytometry: An automated coupling device for rapid sequential flow cytometric sample analysis.

B S Edwards1, F Kuckuck, L A Sklar

  • 1Cancer Research and Treatment Center, Departments of Cytometry and Pathology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA.

Cytometry
|September 16, 1999
PubMed
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This study introduces a novel system for high throughput flow cytometry, enabling repetitive sampling from open vessels without pressure. This plug-flow approach enhances sample delivery efficiency for various screening applications.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Fluid Dynamics

Background:

  • High throughput flow cytometry is often limited by the need for pressurized samples.
  • Existing methods require complex setups for sample handling.

Purpose of the Study:

  • To develop a simple, automated system for repetitive sample delivery from unpressurized sources.
  • To overcome limitations in current flow cytometry sample handling.

Main Methods:

  • A computer-controlled syringe loads samples into a loop within a reciprocating eight-way valve.
  • Sample plugs are transported to the flow cytometer via a pressure-driven fluid line.
  • A second multi-port valve enables sequential delivery from multiple vessels.

Main Results:

Related Experiment Videos

  • The system achieves sample delivery rates up to 9 samples per minute.
  • Uniform sample delivery with reproducible coefficient of variation (CV) was observed.
  • Minimal carryover between samples was noted, even at high rates.

Conclusions:

  • Plug-flow flow cytometry offers a potential solution for automating small sample delivery from unpressurized sources.
  • The developed system is compatible with high-throughput screening and assay applications.
  • This method improves efficiency and accessibility in flow cytometry workflows.