Array biosensor for simultaneous identification of bacterial, viral, and protein analytes

Area of Science:

• Biosensor technology
• Immunotechnology
• Analytical chemistry

Background:

• Simultaneous analysis of multiple analytes is crucial for efficient diagnostics.
• Existing methods often lack the capacity for high-throughput, multiplexed detection.
• Array-based biosensors offer a promising platform for multiplexed analyte quantification.

Purpose of the Study:

• To develop and validate an array biosensor for simultaneous detection of diverse analytes.
• To assess the sensor's performance in analyzing multiple samples and analyte mixtures.
• To determine the sensitivity and detection limits for specific viral, bacterial, and protein targets.

Main Methods:

• Fabrication of a planar waveguide array biosensor utilizing a sandwich immunoassay.
• Immobilization of antigen-specific capture antibodies on the sensor surface.
• Detection of bound analytes using fluorescent tracer antibodies.
• Analysis of 126 blind samples and mixtures of analytes.

Main Results:

• The array biosensor successfully detected viral, bacterial, and protein analytes simultaneously.
• Assay demonstrated high sensitivity, comparable to standard ELISA methods, within a 14-minute timeframe.
• Achieved limits of detection: 10(5) cfu/mL for Bacillus globigii, 10(7) pfu/mL for MS2 bacteriophage, and 10 ng/mL for staphylococcal enterotoxin B (SEB).

Conclusions:

• The developed array biosensor enables rapid, multiplexed detection of multiple analyte classes.
• The sensor is capable of analyzing multiple samples concurrently, offering a significant advantage in diagnostic throughput.
• This technology shows potential for diverse applications requiring simultaneous detection of various targets.