Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium imaging with chemiluminescence.

R Créton1, J A Kreiling, L F Jaffe

  • 1Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA. rcreton@mbl.edu

Microscopy Research and Technique
|September 30, 1999
PubMed
Summary

Aequorin-based calcium imaging offers extreme sensitivity for exploratory studies, enabling detection of broad calcium ranges and long-term recordings. For high spatial resolution, fluorescent imaging methods are recommended.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High Calcium Zones at the Poles of Developing Medaka Eggs.

The Biological bulletin·2018
Same author

Calcium Buffer Injections Block Cytokinesis in Xenopus Eggs.

The Biological bulletin·2018
Same author

Towards the Development of a Technique for Introducing Aequorin into Amoebae of Dictyostelium discoideum Via Electroporation.

The Biological bulletin·2018
Same author

A Region of Steady High Calcium at the Vegetal Pole of Medaka Eggs.

The Biological bulletin·2018
Same author

KCl-Induced Calcium Rise in Squid Eggs: Measurement of Fura-2 Fluorescence.

The Biological bulletin·2018
Same author

Differences in Free Calcium Concentration Between Oocytes and Nurse Cells Revealed by Corrected Aequorin Luminescence.

The Biological bulletin·2018

Area of Science:

  • Biochemistry
  • Cell Biology
  • Imaging Technology

Background:

  • Free cytosolic calcium concentration is a critical second messenger in cellular signaling pathways.
  • Chemiluminescent proteins, such as aequorins, provide a sensitive method for detecting intracellular calcium dynamics.
  • Advancements in imaging systems have improved the ability to visualize cellular processes.

Purpose of the Study:

  • To review the application of chemiluminescent aequorins for imaging free cytosolic calcium.
  • To provide practical guidance on using aequorins for calcium imaging experiments.
  • To compare aequorin-based imaging with fluorescent methods.

Main Methods:

  • Discussion of basic chemiluminescence principles and aequorin biochemistry.
  • Practical advice on aequorin handling and microinjection techniques.

Related Experiment Videos

  • Overview of available ultra-low light imaging systems for aequorin detection.

    • Main Results:

    • Aequorin-based calcium imaging is highly sensitive and suitable for detecting a wide range of calcium concentrations.
    • This method allows for continuous monitoring of calcium levels over extended periods.
    • Aequorin imaging is particularly advantageous for exploratory research due to its sensitivity and broad dynamic range.

    Conclusions:

    • Aequorin-based calcium imaging is the preferred method for initial exploratory studies requiring high sensitivity and long recording times.
    • While aequorins excel in sensitivity, fluorescent imaging techniques are necessary to achieve high spatial resolution in calcium imaging.
    • The choice of imaging method should be tailored to the specific research question, balancing sensitivity with spatial detail.