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Related Experiment Videos

The Shh signalling pathway in early tooth development.

Z Hardcastle1, C C Hui, P T Sharpe

  • 1Department of Craniofacial Development, GKT Dental Institute, Kings College, Guy's Hospital, London, UK.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|October 8, 1999
PubMed
Summary

Sonic Hedgehog (Shh) signalling is crucial for mammalian tooth development. This study reveals Shh pathway gene expression patterns and proposes how Gli-2 regulates cell proliferation during tooth formation.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The Sonic Hedgehog (Shh) signalling pathway is implicated in mammalian tooth development.
  • Understanding Shh pathway gene expression is key to elucidating its role in odontogenesis.

Purpose of the Study:

  • To describe the spatial and temporal expression of Shh pathway genes during early mammalian tooth development.
  • To investigate the roles of Shh signalling as both an epithelial and mesenchymal signal.
  • To propose a mechanism for Gli-2's regulation of cell proliferation in tooth development.

Main Methods:

  • Analysis of spatial and temporal gene expression patterns.
  • Study of Gli-2 mutants and the Fibroblast Growth Factor (Fgf) signalling pathway.

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Main Results:

  • Ptc and Ptch-2, Shh receptors, exhibit distinct cellular localization, suggesting differential Shh signalling.
  • Gli-2 mutants were analyzed in conjunction with the Fgf signalling pathway.

Conclusions:

  • Shh signalling likely functions distinctly in epithelial and mesenchymal compartments during tooth development.
  • A mechanism is proposed for Gli-2's regulation of cell proliferation in tooth development, potentially involving the Fgf pathway.