A spin filter method for continuous evaluation of hemolysis
Related Experiment Video
Updated: Oct 19, 2026
Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
Published on: March 9, 2013
F Alvarez-Núñez1, D A Rosin, S H Yalkowsky
1Department of Pharmacy Practice and Science College of Pharmacy The University of Arizona Tucson, Arizona 85721, USA.
Abstract:
A novel method for quantifying hemolysis is described. This method uses a spin filter to separate the free hemoglobin from the red blood cells suspended in the test solution. This procedure enables the use of a closed loop system that continuously measures hemolysis spectrophotometrically. It is shown that hemolysis does not always stop after the solution has been quenched with normal saline. In fact, the process of hemolysis induced by chemicals such as potassium oleate is relatively slow.
