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Related Experiment Videos

Interferon-gamma increases CD62L expression on human eosinophils.

T Momose1, Y Okubo, S Horie

  • 1First Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.

International Archives of Allergy and Immunology
|October 26, 1999
PubMed
Summary
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Interferon-gamma (IFN-gamma) uniquely increases L-selectin (CD62L) expression on eosinophils, unlike other cytokines. This suggests protein synthesis and cAMP are involved in IFN-gamma

Area of Science:

  • Immunology
  • Cell Biology
  • Hematology

Background:

  • L-selectin (CD62L) and Mac-1 (CD11b/CD18) are critical for eosinophil infiltration.
  • Cytokine-induced changes in eosinophil CD62L and CD11b expression require further investigation.

Purpose of the Study:

  • To investigate the effects of key cytokines (IL-5, GM-CSF, IFN-gamma) on CD62L and CD11b expression in human eosinophils.
  • To examine the impact of dexamethasone, cycloheximide, and theophylline on IFN-gamma-induced CD62L expression.

Main Methods:

  • Human peripheral blood eosinophils were isolated and cultured with IL-5, GM-CSF, or IFN-gamma.
  • Flow cytometry was used to analyze CD62L and CD11b expression after incubation.
  • The modulatory effects of dexamethasone, cycloheximide, and theophylline on CD62L were assessed.

Related Experiment Videos

Main Results:

  • IL-5 and GM-CSF downregulated CD62L and upregulated CD11b expression.
  • IFN-gamma time- and dose-dependently upregulated CD62L expression, with no effect on CD11b.
  • Dexamethasone, cycloheximide, and theophylline reduced CD62L expression on IFN-gamma-stimulated eosinophils.

Conclusions:

  • IFN-gamma uniquely enhances CD62L expression on human eosinophils.
  • Protein synthesis and intracellular cAMP levels are implicated in the IFN-gamma-mediated increase of L-selectin expression.