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Related Experiment Video

Updated: Jul 27, 2026

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice

Published on: July 16, 2014

Brain-derived TNFalpha mediates neuropathic pain.

T A Ignatowski¹, W C Covey, P R Knight

¹Department of Pathology, State University of New York at Buffalo, 14214, USA.

|November 5, 1999

Summary

Brain-derived tumor necrosis factor (TNF) plays a key role in neuropathic pain. Blocking TNF in the brain reduces pain hypersensitivity, suggesting a central role for TNF in pain perception.

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Last Updated: Jul 27, 2026

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Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

Published on: October 6, 2022

Area of Science:

Neuroscience
Pain Research
Molecular Biology

Background:

Neuropathic pain involves central mechanisms following nerve injury, but these are not fully understood.
Tumor necrosis factor (TNF) is implicated in pain pathways.
The role of brain-associated TNF in neuropathic pain requires further investigation.

Purpose of the Study:

To investigate the role of brain-associated TNF in the development of hyperalgesia in the chronic constriction injury (CCI) model of neuropathic pain.
To assess the impact of TNF modulation in the brain on pain perception and hippocampal noradrenergic neurotransmission.

Main Methods:

Rats underwent sciatic nerve ligation (CCI model) to induce neuropathic pain.
TNF levels in brain, spinal cord, and plasma were measured.
Intracerebroventricular (i.c.v.) microinfusion of TNF-antibodies or recombinant TNF-alpha was administered.

Main Results:

Bioactive TNF levels were elevated in the hippocampus following CCI.
Hippocampal TNF elevation correlated with decreased norepinephrine release.
i.c.v. infusion of TNF-antibodies abolished hyperalgesia when started early post-injury.
i.c.v. infusion of TNF-alpha exacerbated hyperalgesia and decreased hippocampal adrenergic neurotransmission.

Conclusions:

Brain-derived TNF is crucial in the pathology of neuropathic pain.
Central TNF signaling contributes to pain hypersensitivity and altered neurotransmission.
Targeting brain TNF may offer a therapeutic strategy for neuropathic pain.