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Related Experiment Videos

Expression pattern of Dkk-1 during mouse limb development.

L Grotewold1, T Theil, U Rüther

  • 1Lehrstuhl für Entwicklungs- und Molekularbiologie der Tiere, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, 40225, Düsseldorf, Germany.

Mechanisms of Development
|November 24, 1999
PubMed
Summary

Dickkopf-1 (Dkk-1) is crucial for head induction and its expression pattern in mouse embryos reveals dynamic roles during limb development. Dkk-1 mRNA is found in developing limb buds, including interdigital mesenchyme, and overlaps with Shh signaling.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Dickkopf-1 (Dkk-1) is a secreted protein essential for head induction in Xenopus by antagonizing Wnt signaling.
  • Dkk-1 homologs are conserved across species, with mouse Dkk-1 expressed early in embryonic development.
  • Previous studies established Dkk-1's role in early embryonic patterning, but its function in limb development was less understood.

Purpose of the Study:

  • To investigate the dynamic expression pattern of Dickkopf-1 (Dkk-1) mRNA during mouse limb development.
  • To determine the spatial and temporal distribution of Dkk-1 in developing limb buds from embryonic day 9.0 to 14.5.
  • To explore the relationship between Dkk-1 expression and key signaling molecules during limb morphogenesis.

Main Methods:

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  • Whole-mount in situ hybridization was used to analyze Dkk-1 mRNA distribution in mouse embryos.
  • Double whole-mount in situ hybridization was employed to examine co-expression patterns with other genes.
  • Analysis focused on limb bud development stages from embryonic day 9.0 to 14.5.

    • Main Results:

    • Dkk-1 expression initiates with forelimb budding and precedes hindlimb budding.
    • Expression becomes localized to two distinct mesenchymal domains by embryonic days 10.5-11.5.
    • Dkk-1 expression domains show initial overlap with Sonic hedgehog (Shh) in the posterior limb bud, later separating, and is restricted to interdigital mesenchyme by embryonic days 12.5-14.5.

    Conclusions:

    • Dkk-1 exhibits a highly dynamic expression pattern throughout mouse limb development.
    • Its expression correlates with key stages of limb bud formation and patterning.
    • The dynamic expression and overlap with Shh suggest a significant role for Dkk-1 in limb morphogenesis and signaling.