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Fluorescent dyes for lymphocyte migration and proliferation studies.
1Division of Immunology and Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia. Christopher.Parish@anu.edu.au
Immunology and Cell Biology
|November 26, 1999
Summary
This review highlights fluorescent dyes for tracking lymphocyte migration and proliferation. Carboxyfluorescein diacetate succinimidyl ester (CFSE) and PKH26 are most versatile for long-term studies and cell division tracking.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Fluorescent dyes are crucial tools for monitoring lymphocyte dynamics.
- Understanding lymphocyte migration and proliferation is vital in immunology and disease research.
Purpose of the Study:
- To review the properties and performance of 14 fluorescent dyes used for lymphocyte tracking over 20 years.
- To identify the most effective dyes for long-term lymphocyte migration and proliferation studies.
Main Methods:
- Comparative analysis of 14 fluorescent dyes based on their performance in tracking lymphocyte migration and proliferation.
- Evaluation of dye characteristics including staining homogeneity, fluorescence intensity halving with cell division, and suitability for in vitro and in vivo applications.
Main Results:
- Carboxyfluorescein diacetate succinimidyl ester (CFSE) and PKH26 are identified as the most versatile dyes for long-term lymphocyte tracking and proliferation quantification.
- CFSE offers superior lymphocyte staining homogeneity and cost-effectiveness compared to PKH26.
- Hoechst 33342 and calcein are suitable for short-term (up to 3 days) lymphocyte migration studies.
Conclusions:
- CFSE and PKH26 are highly effective for tracking lymphocyte division in vitro and in vivo.
- Future developments may yield new fluorescent dyes with improved spectral properties and lymphocyte labeling capabilities.