Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
Kidney Transplant II: Surgical Procedure01:26

Kidney Transplant II: Surgical Procedure

Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living donor...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Application of PCR to the identification of dermatophyte fungi.

Journal of medical microbiology·2000
Same author

WebCat: the library catalogue on the world wide web.

Annals of the Royal College of Surgeons of England·2000
Same author

Mouse mammary tumor virus-Ki-rasB transgenic mice develop mammary carcinomas that can be growth-inhibited by a farnesyl:protein transferase inhibitor.

Cancer research·2000
Same author

Importance of phenylalanine 107 in agonist recognition by the 5-hydroxytryptamine(3A) receptor.

Molecular pharmacology·2000
Same author

Practical application of ergonomic settings of typical computerised workstations.

International journal of occupational safety and ergonomics : JOSE·2000
Same author

Structural basis of DOTMA for its high intravenous transfection activity in mouse.

Gene therapy·2000

Related Experiment Video

Updated: Jul 12, 2026

Murine Kidney Transplant Technique
08:58

Murine Kidney Transplant Technique

Published on: October 20, 2015

Improved techniques for kidney transplantation in the monkey.

S Qi1, J Peng, D Xu

  • 1Laboratory of Experimental Surgery, Research Center of CHUM, Notre-Dame Hospital, University of Montreal, Montreal, Quebec, Canada.

Microsurgery
|December 10, 1999
PubMed
Summary

This study details improved microsurgical techniques for kidney transplantation in monkeys, establishing a reproducible primate model. This advancement aids research into new immunosuppressants and transplantation tolerance mechanisms.

More Related Videos

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach
09:15

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Published on: May 7, 2019

A Modified Surgical Technique for Kidney Transplantation in Mice
10:38

A Modified Surgical Technique for Kidney Transplantation in Mice

Published on: July 22, 2022

Related Experiment Videos

Last Updated: Jul 12, 2026

Murine Kidney Transplant Technique
08:58

Murine Kidney Transplant Technique

Published on: October 20, 2015

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach
09:15

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Published on: May 7, 2019

A Modified Surgical Technique for Kidney Transplantation in Mice
10:38

A Modified Surgical Technique for Kidney Transplantation in Mice

Published on: July 22, 2022

Area of Science:

  • Nephrology
  • Surgical Innovation
  • Primate Research

Background:

  • Kidney transplantation is a critical treatment for end-stage renal disease.
  • Developing reliable animal models is essential for advancing transplantation research.
  • Microsurgical techniques require continuous refinement for improved outcomes.

Purpose of the Study:

  • To describe and validate improved microsurgical techniques for kidney transplantation in a primate model.
  • To establish a reproducible surgical model for preclinical transplantation studies.
  • To support the investigation of immunosuppression and tolerance in transplantation.

Main Methods:

  • Kidney transplantation performed in bilateral nephrectomized Vervet monkeys.
  • Specific techniques include end-to-side renal artery to aorta anastomosis (no aortic patch), renal vein to inferior vena cava anastomosis, and end-to-end ureter anastomosis.
  • Utilized 8-0 nylon sutures for all anastomoses.

Main Results:

  • Successfully performed 60 kidney transplantations in Vervet monkeys.
  • Achieved zero mortality related to surgical procedures or complications.
  • Demonstrated a reproducible and reliable microsurgical technique.

Conclusions:

  • The described microsurgical techniques provide a robust and reproducible model for kidney transplantation in primates.
  • This model is valuable for preclinical testing of novel immunosuppressive agents.
  • Facilitates research into the mechanisms of drug-induced tolerance and xenotransplantation in primates, supporting clinical trials.