Inhibition and recovery of GM-CSF production in the lung after hyperthermia

The purpose of this work was to study the response that is invoked by hyperthermia in the production of Granulocyte-Macrophage colony-stimulating factor (GM-CSF) by the lung. Rats were heated regionally at chest area by a RF generator operating at 27.397 MHz for 1 h at various temperatures and then allowed to recover or repair in various periods of time after heat application. Lung tissue from these animals was then removed and cultured for the production of GM-CSF. GM-CSF was assayed by the production of colonies in the semi-solid agar cultures of bone marrow cells. Immediately after heat treatment hyperthermia had no significant effect on the production of GM-CSF by the lung. A delayed effect was observed about 3.5 h after heat treatment. This effect consisted of a temperature dependent decrease in GM-CSF production. The damage was recoverable and required 1-50 days of post heat treatment time for animals to reach normal level of GM-CSF production. The results suggest that in-vivo application of hyperthermia invokes temporary reduction in GM-CSF production by the lung, which has not been reported previously.