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Related Experiment Videos

Selectin-carbohydrate interactions in shear flow.

M B Lawrence1

  • 1Department of Biomedical Engineering, University of Virginia, Health Sciences Center, Box 377, Charlottesville, VA 22908, USA. mbl2a@virginia.edu.

Current Opinion in Chemical Biology
|December 22, 1999
PubMed
Summary
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Mechanical forces impact selectin bonds, affecting their strength and how long they last. Modifying sialyl Lewis(x) can strengthen L-selectin bonds without changing how they recognize targets, separating mechanical and recognition properties.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Immunology

Background:

  • Selectin bonds mediate cell adhesion, crucial for immune responses.
  • Hydrodynamic shear forces play a role in modulating selectin-mediated interactions.
  • Sialyl Lewis(x) is a key carbohydrate ligand for selectins.

Purpose of the Study:

  • To investigate the interplay between mechanical forces and the biochemical properties of selectin bonds.
  • To determine if chemical modifications of sialyl Lewis(x) can alter L-selectin bond strength independently of recognition.
  • To explore the influence of sulfate orientation on L-selectin bond formation kinetics.

Main Methods:

  • Utilizing techniques to apply controlled hydrodynamic shear stress to selectin bonds.
  • Employing chemical modifications of the sialyl Lewis(x) ligand.

Related Experiment Videos

  • Analyzing changes in L-selectin bond affinity, dissociation rates, and formation rates.

    • Main Results:

    • Hydrodynamic shear stress modulates the mechanical properties (affinity, kinetics) of selectin bonds.
    • Chemical modification of sialyl Lewis(x) enhanced L-selectin bond strength without affecting ligand recognition.
    • Sulfate orientation in sulfo sialyl Lewis(x) was identified as a potential key factor influencing L-selectin bond formation rates.

    Conclusions:

    • Mechanical and biorecognition characteristics of selectin bonds are separable.
    • Chemical modifications offer a route to tune selectin bond mechanics.
    • Sulfate group orientation is a critical determinant of L-selectin bond kinetics, with implications for cell adhesion.