Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interactions between bcl-2 and the IGF system control apoptosis in the developing mouse brain.

N L Baker1, V Carlo Russo, O Bernard

  • 1Centre for Hormone Research, Royal Children's Hospital, Flemington Road, Parkville, Victoria, Australia.

Brain Research. Developmental Brain Research
|December 28, 1999
PubMed
Summary

The Insulin-like Growth Factor (IGF) system and Bcl-2 proteins protect developing brain cells from apoptosis. Their interaction enhances neuronal survival, particularly in the olfactory bulb and cerebellum, suggesting a robust protective mechanism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum IGFBP-2 levels are associated with reduced insulin sensitivity in obese children.

Clinical obesity·2018
Same author

Effects of yohimbine and drug cues on impulsivity and attention in cocaine-dependent men and women and sex-matched controls.

Drug and alcohol dependence·2016
Same author

Growth and the Growth Hormone-Insulin Like Growth Factor 1 Axis in Children With Chronic Inflammation: Current Evidence, Gaps in Knowledge, and Future Directions.

Endocrine reviews·2016
Same author

IGFBP-2: The dark horse in metabolism and cancer.

Cytokine & growth factor reviews·2014
Same author

IGFBP-2 inhibits adipogenesis and lipogenesis in human visceral, but not subcutaneous, adipocytes.

International journal of obesity (2005)·2014
Same author

IGFBP-2 nuclear translocation is mediated by a functional NLS sequence and is essential for its pro-tumorigenic actions in cancer cells.

Oncogene·2013

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • The Insulin-like Growth Factor (IGF) system and Bcl-2 proteins are crucial for preventing apoptosis during brain development.
  • Understanding the interplay between these anti-apoptotic systems is vital for comprehending neural development and repair.

Purpose of the Study:

  • To investigate the relationship between the IGF system and Bcl-2 proteins in protecting developing neurons from apoptosis.
  • To examine how overexpressing Bcl-2 or IGF-I affects neuronal survival and organization in specific brain regions.

Main Methods:

  • Utilized transgenic mice models overexpressing Bcl-2 in olfactory bulb (OB) neurons and IGF-I in cerebellar neurons.
  • Cultured OBs in serum-free medium to induce apoptosis and assessed neuronal survival and organization.

Related Experiment Videos

  • Analyzed protein expression and immunoreactivity for IGF-I, IGFBP-2, and Bcl-2.

    • Main Results:

    • Bcl-2 overexpression preserved mitral cells in the OB, and IGF-I further improved OB layer organization and neuronal survival in both wildtype and Bcl-2 transgenic mice.
    • IGF-I and IGFBP-2 immunoreactivity increased in Bcl-2-expressing mitral cells.
    • IGF-I overexpression in cerebellar Purkinje cells led to increased Bcl-2 and IGFBP-2 immunoreactivity.

    Conclusions:

    • IGF-I modulates the expression of its binding protein IGFBP-2 and the anti-apoptotic protein Bcl-2 in the developing brain.
    • Bcl-2 expression inhibits apoptosis in OB mitral neurons, associated with enhanced IGF system components.
    • The interaction between the IGF system and Bcl-2 proteins provides a robust cell protection mechanism during brain development and repair.