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Updated: Aug 11, 2026
Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
Published on: May 27, 2015
beta-galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and
1Department of Chemistry, University of Winnipeg, 515 Portage Avenue, Winnipeg, Manitoba, Canada R3B 2E9.
Abstract:
beta-Galactosidase was incubated for 60 min with the fluorogenic substrate resorufin-beta-D-galactopyranoside, which is converted by the action of the enzyme into resorufin and galactose. A 160 pL aliquot of reaction mixture was analyzed by capillary electrophoresis utilizing laser-induced fluorescence detection. Based on the detection of the resorufin formed, the limit of detection of beta-galactosidase was 1.5 x 10(-15) M or 900 molecules of enzyme in a 1 microL sample.
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