Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mass spectrometry. From genomics to proteomics.

J R Yates1

  • 1Department of Molecular Biotechnology, University of Washington, Seattle 98195-7730, USA. jyates@u.washington.edu

Trends in Genetics : TIG
|January 19, 2000
PubMed
Summary

Large-scale DNA sequencing advances proteomics by enabling rapid, automated protein identification using mass spectrometry. This high-throughput approach is crucial for understanding protein networks in biological processes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Lorentz sphere visualised.

The Journal of chemical physics·2019
Same author

Lonomia obliqua bristle extract modulates Rac1 activation, membrane dynamics and cell adhesion properties.

Toxicon : official journal of the International Society on Toxinology·2019
Same author

Individual differences in impulsive action and dopamine transporter function in rat orbitofrontal cortex.

Neuroscience·2015
Same author

ProLuCID: An improved SEQUEST-like algorithm with enhanced sensitivity and specificity.

Journal of proteomics·2015
Same author

Global quantitative analysis of phosphorylation underlying phencyclidine signaling and sensorimotor gating in the prefrontal cortex.

Molecular psychiatry·2015
Same author

Effects of methylprednisolone and 4-chloro-3-hydroxyanthranilic acid in experimental spinal cord injury in the guinea pig appear to be mediated by different and potentially complementary mechanisms.

Spinal cord·2014

Area of Science:

  • Proteomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Genomic data provides a foundation for protein analysis.
  • Advancements in mass spectrometry enable high-throughput protein identification.

Purpose of the Study:

  • To highlight how DNA sequencing infrastructure supports proteomics.
  • To describe the impact of mass spectrometry on protein identification.
  • To explain the role of these advancements in studying protein networks.

Main Methods:

  • Utilizing large-scale DNA sequencing data.
  • Employing mass spectrometry for protein data generation.
  • Searching protein and nucleotide sequence databases for identification.

Main Results:

  • Enabled rapid and automated protein identification.
  • Facilitated high-throughput and large-scale protein analysis.
  • Advanced protein expression studies and identification of proteins in complexes.

Conclusions:

  • Technological integration of DNA sequencing and mass spectrometry accelerates proteomic research.
  • These methods are essential for deciphering complex biological protein networks.

Related Experiment Videos