Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electroosmosis- and pressure-driven chromatography in chips using continuous beds.

C Ericson1, J Holm, T Ericson

  • 1Department of Biochemistry, University of Uppsala, Sweden.

Analytical Chemistry
|February 3, 2000
PubMed
Summary

Microchip chromatography, utilizing continuous polymer beds, enhances separation efficiency for diverse molecules. This innovative technique eliminates wall effects and simplifies equipment design for automated analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The continuous reaction time test for minimal hepatic encephalopathy validated by a randomized controlled multi-modal intervention-A pilot study.

PloS one·2017
Same author

Defibrillation before EMS arrival in western Sweden.

The American journal of emergency medicine·2017
Same author

The effect of amiodarone on warfarin anticoagulation: a register-based nationwide cohort study involving the Swedish population.

Journal of thrombosis and haemostasis : JTH·2017
Same author

Regional contribution to ventricular stroke volume is affected on the left side, but not on the right in patients with pulmonary hypertension.

The international journal of cardiovascular imaging·2016
Same author

Breast cancer genetic risk profile is differentially associated with interval and screen-detected breast cancers.

Annals of oncology : official journal of the European Society for Medical Oncology·2016
Same author

Comparative Investigation of Postoperative Complications in Patients With Gastroesophageal Junction Cancer Treated With Preoperative Chemotherapy or Surgery Alone.

Scandinavian journal of surgery : SJS : official organ for the Finnish Surgical Society and the Scandinavian Surgical Society·2015

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Microfluidics

Background:

  • Traditional chromatography often faces limitations due to "wall effects" and the need for supporting frits.
  • Microchip-based separation techniques offer potential for miniaturization and automation.

Purpose of the Study:

  • To demonstrate the extension of microchip capabilities to various chromatography modes using continuous polymer beds.
  • To eliminate wall effects and the requirement for frits in microchip chromatography.
  • To assess the performance of microchip chromatography for both low- and high-molecular-weight analytes.

Main Methods:

  • Filling microchip channels with continuous polymer beds anchored to the channel walls.
  • Utilizing a quartz chip with integrated auxiliary components for flexible automation.

Related Experiment Videos

  • Performing chromatographic separations in both fused-silica capillaries and microchips.

    • Main Results:

    • Elimination of "wall effects" by anchoring polymer beds to channel walls.
    • Successful separation of low-molecular-weight (alkyl phenones, antidepressants) and high-molecular-weight substances (proteins) with comparable resolution to capillaries.
    • Rapid electrochromatographic separation of uracil, phenol, and benzyl alcohol in under 20 seconds.

    Conclusions:

    • Continuous polymer beds in microchips provide an effective alternative to traditional chromatography, eliminating wall effects and frits.
    • The flexible, automated design of the quartz chip system is suitable for diverse analytical applications.
    • Microchip chromatography demonstrates high performance for a wide range of analytes, enabling rapid separations.