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Apparatus and method for interfacing capillary electrophoresis and chemiluminescence nitrogen detection for the
A A Mohammad1, E Fujinari, A O Okorodudu
1Department of Pathology, University of Texas-Medical Branch, Galveston 77555-0551, USA.
Journal of Chromatography. A
|February 17, 2000
Abstract:
We have developed an interface that allows the specific detection of nitrogen-containing compounds by using a chemiluminescence nitrogen detector. The feasibility of using this interface was demonstrated by separating and detecting two nitrogen-containing compounds, p-aminosalicylic acid and L-phenylalanine. Although baseline separation was achieved, the theoretical plates were lower when compared to UV detection (25000 vs. approximately 85000). A sensitivity of 75 ng (approximately 500 pmol) per injection was achieved with this system which is adequate for pharmaceutical and biotech applications.