[Identification of antigens shared between Schistosoma japonicum and Trichinella spiralis]

Cross-reactivity was analysed between the antigen of T. spiralis muscular larvae and antigens of cercariae, liver stage schistosomula, 30 day male and female adult worms of S. japonicum by means of EITB technique. The result showed that the antigens of cercariae schistosomula, female worms except male worms, were recognized by sera from rabbits immunized with T. spiralis antigens or by sera from T. spiralis infected rabbits. The molecular weight of recognized antigens ranges from 15 to 100 kD, mainly from 50 to 70 kD. The immunized rabbit sera and the infected rabbit sera reacted with the same cross-antigens of S. japonicum. Among these schistosome stages, the cercariae showed the most cross-reactive bands, the second were the liver stage schistosomula, and 30 day female worms only showed a weak band which located at 97 kD position. Meanwhile, the anti-S. japonicum sera recognized several T. spiralis antigens which located at 34 kD and above, especially 35-38 kD. The results suggest that there are many antigens shared between T. spiralis and S. japonicum and the main cross-reactive antigens are found in the larva stages of S. japonicum and T. spiralis muscular larva. The cross-reactive antigens are different between various stages of S. japonicum and muscular larva of T. spiralis.