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Related Experiment Videos

Regulation of RAG expression in developing lymphocytes.

H Nagaoka1, W Yu, M C Nussenzweig

  • 1Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA.

Current Opinion in Immunology
|March 14, 2000
PubMed
Summary

Green fluorescent protein (GFP)-RAG indicator mice allow in vivo visualization of recombination-activating gene (RAG) expression. This advance helps define RAG-expressing cells in lymphoid organs and reveals cis requirements for RAG regulation.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Proper expression of recombination-activating genes (RAGs) is crucial for adaptive immune system development.
  • Understanding RAG gene regulation is key to deciphering immune cell development.
  • Previous methods limited in vivo analysis of RAG expression dynamics.

Purpose of the Study:

  • To establish and utilize novel green fluorescent protein (GFP)-RAG indicator mouse strains for in vivo RAG expression analysis.
  • To define the specific cell types and locations of RAG expression within secondary lymphoid organs.
  • To investigate the cis regulatory elements controlling stage- and lineage-specific RAG expression.

Main Methods:

  • Generation of genetically engineered mouse models expressing GFP fused to RAG proteins.

Related Experiment Videos

  • In vivo imaging and flow cytometry of cells from secondary lymphoid organs.
  • Analysis of RAG expression patterns in different immune cell populations and developmental stages.

    • Main Results:

    • Successful establishment of GFP-RAG indicator mouse strains enabling real-time RAG expression monitoring.
    • Identification of specific immune cell subsets within secondary lymphoid organs that express RAGs.
    • Demonstration of distinct cis regulatory requirements for RAG expression depending on cell stage and lineage.

    Conclusions:

    • GFP-RAG indicator mice are powerful tools for studying adaptive immunity and RAG regulation.
    • In vivo visualization provides critical insights into the cellular and molecular control of RAG expression.
    • Findings highlight the complex regulatory mechanisms governing RAG expression during immune cell development.