Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

99Tcm-MAG3: problems with radiochemical purity testing.

T Murray1, K McKellar, J Owens

  • 1Radionuclide Dispensary, Western Infirmary, Glasgow, UK. murraythomas@hotmail.com

Nuclear Medicine Communications
|March 16, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing acute thermal assays as a rapid screening tool for coral restoration.

Scientific reports·2024
Same author

The importance of practical skills.

British dental journal·2023
Same author

Accuracy of Point-of-Care-Ultrasonography in Confirming Shoulder Reduction in Emergency Departments.

Irish medical journal·2022
Same author

Evaluating the performance of the GeneXpert HIV-1 qualitative assay as a consecutive test for a new early infant diagnosis algorithm in South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2021
Same author

An automated approach to determine antibody endpoint titers for COVID-19 by an enzyme-linked immunosorbent assay.

Immunohematology·2021
Same author

Serum pooling for rapid expansion of anti-SARS-CoV-2 antibody testing capacity.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2020
Same journal

Diagnostic performance and quantitative characterization of PET/computed tomography in peritoneal deposits assessment.

Nuclear medicine communications·2026
Same journal

Diagnostic value of dual-time-point 18F-fluorodeoxyglucose PET/computed tomography in differentiating benign and malignant extrahepatic bile duct occupying lesions.

Nuclear medicine communications·2026
Same journal

Prognostic value of baseline 18F- fluroodexoygloucose PET/computed tomography-derived maximum standardized uptake value and tumor bulk in pediatric Hodgkin lymphoma: a retrospective analysis.

Nuclear medicine communications·2026
Same journal

A comparative assessment of [99mTc] HYNIC-PSMA-11 scintigraphy in prostate cancer as a potential substitute for disease evaluation with [68Ga] PSMA-11 PET/CT imaging.

Nuclear medicine communications·2026
Same journal

Prediction of tumor radiation absorbed dose in radionuclide therapy based on the mean standardized uptake value from PET imaging.

Nuclear medicine communications·2026
Same journal

Serum cytokine profiles in papillary thyroid carcinoma patients with lung metastases: characteristics and the relationship with prognosis.

Nuclear medicine communications·2026
See all related articles

Comparing radiochemical purity (RCP) methods for 99Tcm-MAG3, solid-phase extraction (SPE) underestimated RCP, while thin-layer chromatography (TLC) overestimated it, impacting quality control.

Area of Science:

  • Nuclear Medicine
  • Radiopharmaceutical Chemistry
  • Analytical Chemistry

Background:

  • Accurate determination of radiochemical purity (RCP) is crucial for the quality and efficacy of radiopharmaceuticals.
  • 99Tcm-MAG3 is a widely used renal imaging agent, necessitating reliable analytical methods for its quality control.

Purpose of the Study:

  • To compare the accuracy and reliability of solid-phase extraction (SPE) and thin-layer chromatography (TLC) methods for determining the RCP of 99Tcm-MAG3 against high-performance liquid chromatography (HPLC).
  • To identify potential sources of discrepancy between different analytical techniques used for 99Tcm-MAG3 RCP assessment.

Main Methods:

  • Radiochemical purity of 99Tcm-MAG3 was assessed using three distinct analytical techniques: SPE, HPLC, and TLC.
  • Qualitative analysis of SPE fractions was performed using HPLC and TLC to investigate method-specific impurities.

Related Experiment Videos

  • Comparative analysis of results obtained from SPE, TLC, and HPLC was conducted, including statistical evaluation of differences.

    • Main Results:

    • SPE significantly underestimated RCP (86.0 +/- 5.1%) compared to HPLC (94.4 +/- 1.4%), attributed to the co-elution of 99Tcm-MAG3 with impurities.
    • TLC significantly overestimated RCP (98.3 +/- 0.6%) compared to HPLC (95.8 +/- 0.9%), due to the inability to separate a key lipophilic impurity from 99Tcm-MAG3.
    • SPE demonstrated a lack of specificity, leading to the misclassification of fractions and underestimation of RCP.

    Conclusions:

    • The SPE method for 99Tcm-MAG3 RCP determination is unreliable and leads to significant underestimation, potentially causing unnecessary rejection of radiopharmaceutical kits.
    • The TLC method overestimates RCP due to insufficient separation of critical impurities, compromising accurate quality assessment.
    • HPLC is presented as a more reliable method for accurate RCP determination of 99Tcm-MAG3, highlighting the need for careful selection and validation of analytical techniques in radiopharmaceutical quality control.