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AC voltammetric carbon paste-based enzyme immunosensors.

C Fernández-Sánchez1, M B González-García, A Costa-García

  • 1Departamento de Química Física y Analítica, Universidad de Oviedo, Asturias, Spain.

Biosensors & Bioelectronics
|March 18, 2000
PubMed
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This study introduces a novel voltammetric immunosensor using carbon paste electrodes for detecting human IgG. The reusable sensor demonstrates good reproducibility and a low detection limit of 10(-10) M.

Area of Science:

  • Electrochemistry
  • Biosensors
  • Immunochemistry

Background:

  • Carbon paste electrodes (CPEs) are versatile platforms for electrochemical sensing.
  • Developing reusable and sensitive immunosensors is crucial for diagnostics.

Purpose of the Study:

  • To develop a novel voltammetric immunosensor for human IgG detection.
  • To utilize alkaline phosphatase-labeled immunoglobulin and its enzymatic product for signal generation.
  • To establish a reusable sensor platform through electrochemical cleaning.

Main Methods:

  • Anodized carbon paste electrodes were functionalized with immunochemical reagents.
  • Alkaline phosphatase-labeled immunoglobulin was used as a tracer.
  • Enzymatic hydrolysis of 3-indoxyl phosphate produced indigo dimer, detected by AC voltammetry.

Related Experiment Videos

  • Electrochemical anodization was employed for sensor regeneration.

    • Main Results:

    • The immunosensor successfully detected human IgG.
    • Good reproducibility of the electrodic signal was achieved.
    • A limit of detection around 10(-10) M for human IgG was obtained.

    Conclusions:

    • The developed voltammetric immunosensor offers a sensitive and reproducible method for human IgG determination.
    • The sensor's reusability enhances its practical applicability.
    • This approach provides a foundation for developing other immunosensors based on similar principles.