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Updated: Jul 20, 2026
12:11
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
AC voltammetric carbon paste-based enzyme immunosensors
C Fernández-Sánchez1, M B González-García, A Costa-García
1Departamento de Química Física y Analítica, Universidad de Oviedo, Asturias, Spain.
Biosensors & Bioelectronics
|March 18, 2000
Summary
This study introduces a novel voltammetric immunosensor using carbon paste electrodes for detecting human IgG. The reusable sensor demonstrates good reproducibility and a low detection limit of 10(-10) M.
Area of Science:
- Electrochemistry
- Biosensors
- Immunochemistry
Background:
- Carbon paste electrodes (CPEs) are versatile platforms for electrochemical sensing.
- Developing reusable and sensitive immunosensors is crucial for diagnostics.
Purpose of the Study:
- To develop a novel voltammetric immunosensor for human IgG detection.
- To utilize alkaline phosphatase-labeled immunoglobulin and its enzymatic product for signal generation.
- To establish a reusable sensor platform through electrochemical cleaning.
Main Methods:
- Anodized carbon paste electrodes were functionalized with immunochemical reagents.
- Alkaline phosphatase-labeled immunoglobulin was used as a tracer.
- Enzymatic hydrolysis of 3-indoxyl phosphate produced indigo dimer, detected by AC voltammetry.
- Electrochemical anodization was employed for sensor regeneration.
Main Results:
- The immunosensor successfully detected human IgG.
- Good reproducibility of the electrodic signal was achieved.
- A limit of detection around 10(-10) M for human IgG was obtained.
Conclusions:
- The developed voltammetric immunosensor offers a sensitive and reproducible method for human IgG determination.
- The sensor's reusability enhances its practical applicability.
- This approach provides a foundation for developing other immunosensors based on similar principles.
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