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Sequential patterns of inflammatory events during developing and expressed skin late-phase reactions
B Zweiman1, I C Haralabatos, N C Pham
1Allergy and Immunology Section, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania Medical Center, Philadelphia, PA, USA.
The Journal of Allergy and Clinical Immunology
|April 11, 2000
Summary
Late-phase reactions (LPR) involve early accumulation of neutrophils and eosinophils, evolving from immediate wheal responses within six hours. This study details the sequential inflammatory cell events during skin LPR development.
Area of Science:
- Immunology
- Dermatology
- Allergy Research
Background:
- Late-phase reactions (LPR) occur 6-24 hours after allergen exposure.
- The early development of LPR is not well understood.
Purpose of the Study:
- To compare sequential gross and histologic inflammatory responses during the development of skin LPR within six hours after allergen challenge.
Main Methods:
- Seven atopic subjects received intradermal allergen injections.
- Biopsies were taken at 20 minutes, 1, 2, and 6 hours for immunohistochemistry.
- Gross reactions and inflammatory cell accumulation were compared to control sites and 24-hour findings.
Main Results:
- Gross LPR evolved from immediate wheal responses within six hours.
- Neutrophil accumulation peaked at 1 hour, followed by significant eosinophil accumulation by 6 hours.
- T-cell accumulation was most prominent at 24 hours, with early E-selectin expression on vessels.
Conclusions:
- Skin LPR involves early neutrophil and eosinophil influx, distinct from lymphocyte entry.
- The development of induration is linked to early inflammatory cell accumulation.
- The pathogenesis of LPR is complex and likely involves multiple factors.
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