Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Blood-borne cancer cells--quo vadis?

B Brandt1, H Schmitt, J C Feldner

  • 1Institute of Clinical Chemistry, University of Münster, Germany. brandt@uni-muenster.de

The International Journal of Biological Markers
|April 14, 2000
PubMed
Summary

Detecting blood-borne cancer cells aids staging and metastasis understanding. An immunomagnetic method identified cytokeratin-positive cells in prostate and breast cancer patients, correlating with disease stage and treatment response.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Calcium isotope composition in serum and urine for the assessment of bone mineral balance (BMB) - The Osteolabs post-market follow-up study.

Bone·2024
Same author

Transcatheter aortic valve implantation in nonagenarians: insights from the German Aortic Valve Registry (GARY).

Clinical research in cardiology : official journal of the German Cardiac Society·2020
Same author

Symmetry breakdown of electron emission in extreme ultraviolet photoionization of argon.

Nature communications·2018
Same author

Small dense LDL cholesterol in human subjects with different chronic inflammatory diseases.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2018
Same author

[Recommendations for extracorporeal cardiopulmonary resuscitation (eCPR) : Consensus statement of DGIIN, DGK, DGTHG, DGfK, DGNI, DGAI, DIVI and GRC].

Der Anaesthesist·2018
Same author

[Recommendations for extracorporeal cardiopulmonary resuscitation (eCPR) : Consensus statement of DGIIN, DGK, DGTHG, DGfK, DGNI, DGAI, DIVI and GRC].

Medizinische Klinik, Intensivmedizin und Notfallmedizin·2018

Area of Science:

  • Oncology
  • Biotechnology
  • Molecular Diagnostics

Background:

  • Blood-borne cancer cells, or circulating tumor cells (CTCs), are crucial for understanding cancer metastasis and clinical staging.
  • Current methods for detecting CTCs face challenges in sensitivity and specificity.

Purpose of the Study:

  • To develop and validate a cytokeratin-based immunomagnetic method for isolating epithelium-derived CTCs from patient blood.
  • To correlate the presence and quantity of specific CTC clusters with disease stage in prostate and breast cancer patients.
  • To assess the impact of therapy on CTC detection and explore molecular alterations in CTCs.

Main Methods:

  • Development of a cytokeratin-based immunomagnetic separation technique.
  • Immunohistochemical staining for cytokeratin and cancer-specific antigens (PSA for prostate cancer, p185c-erbB-2 for breast cancer).

Related Experiment Videos

  • Sensitive detection of microsatellite alterations using multiplex-PCR.

    • Main Results:

    • The number of cytokeratin/PSA-positive cell clusters in prostate cancer patients and cytokeratin/p185c-erbB-2-positive clusters in breast cancer patients correlated with disease stage.
    • Breast cancer patients receiving adriamycin adjuvant therapy showed a decrease in cytokeratin/p185c-erbB-2-positive cell clusters.
    • Microsatellite multiplex-PCR enabled reproducible detection of microsatellite alterations in CTCs.

    Conclusions:

    • The developed immunomagnetic method effectively isolates and detects CTCs.
    • CTCs can serve as biomarkers for disease staging and monitoring treatment response in prostate and breast cancer.
    • Molecular characterization of CTCs holds promise for personalized diagnostic and therapeutic strategies.