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Comparative study of two ribozymes and DNA-enzyme against the same RNA target
1Department of Ecological Engineering, Toyohashi University of Technology, Aichi, Japan.
Nucleic Acids Symposium Series
|April 26, 2000
Abstract:
Two ribozymes, hammerhead ribozyme and hairpin ribozyme, and a DNA-enzyme were designed to cleave a same RNA target, the same site of the rat complement regulatory factor 512 antigen mRNA. The kinetic properties of these RNA-cleaving enzymes were measured and compared under the same conditions, using multiple turnover kinetics and competition kinetics. The catalytic efficiencies of these enzymes, and also the order of these enzymes will be discussed.