Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Wnt/Shh interactions regulate ectodermal boundary formation during mammalian tooth development.

L Sarkar1, M Cobourne, S Naylor

  • 1Department of Craniofacial Development, GKT Dental Institute, Guy's Hospital, London SE1 9RT, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|April 26, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Establishing an International Psychoanalytic Nurses Alliance [IPNA].

Journal of psychiatric and mental health nursing·2026
Same author

Distinct Involvement of X-Inactivation in Organogenesis.

Journal of dental research·2026
Same author

Computational Methods for Image Analysis in Craniofacial Development and Disease.

Journal of dental research·2024
Same author

Redefining Mucosal Inflammation with Spatial Genomics.

Journal of dental research·2024
Same author

Exploring peer tutoring from the peer tutor's perspective.

Radiography (London, England : 1995)·2022
Same author

Experiences of diagnostic radiographers through the Covid-19 pandemic.

Radiography (London, England : 1995)·2021
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Wingless (Wnt)-7b signaling in oral ectoderm represses Sonic hedgehog (Shh) in dental ectoderm, maintaining boundaries crucial for mammalian tooth development. This interaction ensures proper tooth formation sites.

Area of Science:

  • Developmental Biology
  • Molecular Signaling
  • Ectodermal Development

Background:

  • Wnt and hedgehog signaling pathways are critical for cell boundary formation in Drosophila segmentation.
  • Mammalian tooth development requires precise boundaries between oral and dental ectoderm for correct tooth site positioning.

Purpose of the Study:

  • To investigate the reciprocal relationship between Wnt-7b and Sonic hedgehog (Shh) signaling in mouse tooth development.
  • To elucidate the role of Wnt-7b in maintaining ectodermal boundaries during early tooth formation.

Main Methods:

  • Analysis of Wnt-7b and Shh expression patterns in mouse embryonic mandibular arch explants.
  • Ectopic expression of Wnt-7b using a murine retrovirus in presumptive dental ectoderm.
  • Manipulation of Shh signaling by implanting Shh-soaked beads.

Related Experiment Videos

Main Results:

  • A reciprocal expression pattern of Wnt-7b (oral ectoderm) and Shh (dental ectoderm) was identified, marking ectodermal boundaries.
  • Ectopic Wnt-7b expression led to down-regulation of Shh and Ptc, arresting tooth development.
  • Shh protein rescue experiments confirmed Wnt-7b's specific repression of Shh signaling.

Conclusions:

  • Wnt-7b acts to repress Shh expression, thereby maintaining the distinct boundaries between oral and dental ectodermal cells.
  • This Wnt-7b-mediated repression of Shh signaling is essential for the initiation and proper progression of mammalian tooth development.