Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Suspension arrays for high throughput, multiplexed single nucleotide polymorphism genotyping.

B Armstrong1, M Stewart, A Mazumder

  • 1Axys Pharmaceuticals, La Jolla, CA, USA.

Cytometry
|May 11, 2000
PubMed
Summary

This study introduces a new, cost-effective assay for single nucleotide polymorphism (SNP) genotyping. The method uses microsphere-based suspension arrays for high-throughput genetic diversity analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Australian guidelines for anal cancer screening using anal human papillomavirus testing with cytology triage in people living with HIV.

HIV medicine·2025
Same author

Advances in diagnostic and therapeutic applications of mismatch repair loss in cancer.

DNA repair·2025
Same author

The utility of behavioral biometrics in user authentication and demographic characteristic detection: a scoping review.

Systematic reviews·2024
Same author

Feasibility and preliminary efficacy of structured programming and a parent intervention to mitigate accelerated summer BMI gain: a pilot study.

Pilot and feasibility studies·2023
Same author

Towards next generation digital twin in robotics: Trends, scopes, challenges, and future.

Heliyon·2023
Same author

Correction to: Early-stage studies to larger-scale trials: investigators' perspectives on scaling-up childhood obesity interventions.

Pilot and feasibility studies·2022

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Genetic diversity influences disease susceptibility and drug response.
  • Single nucleotide polymorphisms (SNPs) are the most common genetic variants.
  • Accurate SNP genotyping is crucial for personalized medicine.

Purpose of the Study:

  • To develop a low-cost, high-throughput assay for SNP genotyping.
  • To demonstrate the feasibility of multiplexed genotyping using suspension arrays.

Main Methods:

  • Utilizes oligonucleotide probes attached to fluorescently encoded microspheres.
  • Hybridizes probes directly to fluorescently labeled polymerase chain reaction (PCR) products.
  • Analyzes results using a standard flow cytometer.

Related Experiment Videos

Main Results:

  • Achieved good agreement with TaqMan genotyping methods.
  • Optimized probe length and target concentration enhanced assay performance.
  • Demonstrated a 32-plex assay, simultaneously genotyping eight polymorphic genes.

Conclusions:

  • First demonstration of multiplexed genotyping with suspension arrays and direct hybridization.
  • Offers enhanced flexibility over conventional chips by allowing probe addition/removal.
  • Improves throughput, cost-effectiveness, and speed through multiplexed PCR and hybridization.