Apoptosis induced by hydrogen peroxide under serum deprivation and its inhibition by antisense c-jun in F-MEL cells

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Y H Kim1, M Takahashi, E Suzuki

  • 1Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan. sarah@oxygen.rcast.u-tokyo.ac.jp

Biochemical and Biophysical Research Communications
|May 18, 2000
PubMed

Insights

Serum deprivation induces apoptosis in F-MEL cells via hydrogen peroxide (H2O2) generation. Inhibiting c-jun expression suppressed this H2O2-mediated cell death, highlighting c-jun

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Serum deprivation triggers apoptosis in F-MEL cells.
  • Previous studies indicated that inhibiting c-jun expression suppresses serum deprivation-induced apoptosis.

Purpose of the Study:

  • To elucidate the mechanisms underlying serum deprivation-induced apoptosis.
  • To identify the specific molecule responsible for apoptosis under serum deprivation.
  • To investigate the role of c-jun in hydrogen peroxide-induced apoptosis.

Main Methods:

  • Treatment of F-MEL cells with catalase and N-acetyl-L-cysteine (NAC).
  • Assessment of intracellular hydrogen peroxide (H2O2) generation using Western blot.
  • Induction of apoptosis by direct application of H2O2 and evaluation in c-junAS (+) cells.
  • Confirmation of apoptosis via DNA fragmentation, TUNEL assay, and nuclear morphology.

Main Results:

  • Catalase and NAC treatment suppressed cell death under serum deprivation.
  • Intracellular H2O2 generation was detected in parental F-MEL cells but suppressed in c-junAS (+) cells.
  • Direct H2O2 application induced apoptosis, which was suppressed in c-junAS (+) cells.
  • c-jun expression is essential for H2O2-induced apoptosis.

Conclusions:

  • Apoptosis induced by serum deprivation in F-MEL cells is mediated by hydrogen peroxide (H2O2).
  • c-jun expression is critical for the induction of apoptosis by H2O2 in F-MEL cells.

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