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Updated: Oct 9, 2026

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
Published on: March 24, 2009
Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ
R P van Gijlswijk1, M P van de Corput, V Bezrookove
1Department of Molecular Cell Biology, Leiden University Medical Center, The Netherlands.
Abstract:
A method is presented to conjugate horseradish peroxidase (HRP) to oligodeoxynucleotides for fluorescence in situ hybridization assays employing tyramide signal amplification (TSA). HRP is covalently bound to the oligonucleotide by thiol ether linkage and purified by high-performance liquid chromatography. With TSA detection, a single HRP-labeled oligonucleotide probe is sufficient for in situ detection of clustered DNA repeat sequences with a degree of repetition between 20 and 50.
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