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Leukocyte detection in human semen using flow cytometry.

G Ricci1, G Presani, S Guaschino

  • 1Department of Obstetrics and Gynaecology, University of Trieste, Istituto per l'Infanzia 'Burlo Garofolo', IRCCS, Trieste, Italy. ricci@burlo.trieste.it

Human Reproduction (Oxford, England)
|June 1, 2000
PubMed
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Flow cytometry with monoclonal antibodies offers a reliable method for detecting leukocytes in semen. This advanced technique accurately identifies white blood cells (WBCs) and their subpopulations, improving upon traditional tests.

Area of Science:

  • Reproductive Biology
  • Immunology
  • Clinical Chemistry

Background:

  • Leukocytes in semen (leukocytospermia) can impact male fertility.
  • Traditional methods for detecting leukocytospermia have limitations in sensitivity and specificity.

Purpose of the Study:

  • To establish and validate a flow cytometry method for evaluating leukocytes in semen.
  • To compare the efficacy of this new method against conventional tests.

Main Methods:

  • Flow cytometry combined with monoclonal antibodies (anti-CD45, anti-CD53) was used to analyze ejaculates from 59 males.
  • Routine semen analyses, peroxidase, and polymorphonuclear granulocyte-elastase detection were performed for comparison.

Main Results:

Related Experiment Videos

  • Flow cytometry accurately quantified total leukocytes and identified subpopulations, even at low concentrations.
  • Peroxidase and elastase tests showed lower sensitivity compared to flow cytometry.
  • A lower threshold for leukocytospermia (2x10^5 WBC/ml) using flow cytometry correlated with reduced sperm concentration.

    • Conclusions:

    • Flow cytometry with monoclonal antibodies is a simple, reproducible, and accurate method for detecting leukocytes and their subpopulations in semen.
    • This technique offers improved diagnostic capabilities for assessing male reproductive health compared to conventional methods.