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Fish muscle parvalbumins as marker proteins for native and urea isoelectric focusing
H Rehbein1, R Kündiger, C Pineiro
1Federal Research Center for Fisheries, Institute of Biochemistry and Technology, Hamburg, Germany. rehbein.ibt@bfa-fisch.de
Electrophoresis
|June 1, 2000
Abstract:
An isoelectric point (pI) calibration kit containing fish muscle parvalbumins was prepared and tested for its suitability for isoelectric focusing (IEF) in the presence of 8 M urea. The pattern obtained by urea CleanGel IEF consisted of nine bands covering the pI range 4.96-5.64. This range is relevant for species identification of heated fish by urea IEF. The kit may also be used for native IEF in the low pH range, as demonstrated by running an extract made from the kit together with water-soluble fish muscle proteins on Servalyt Precotes 3-6.