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Related Experiment Videos

Diffusion coefficient and capacity factor in capillary electrokinetic chromatography with replaceable charged

B Maichel1, B Gas, E Kenndler

  • 1Institute for Analytical Chemistry, University of Vienna, Austria.

Electrophoresis
|June 1, 2000
PubMed
Summary
This summary is machine-generated.

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Tribute to J.F.K. Huber.

Fresenius' journal of analytical chemistry·2001

Apparent diffusion coefficients for neutral analytes decrease with increasing poly(diallyidimethylammonium) concentration, indicating analyte partitioning interactions in electrokinetic chromatography.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Physical Chemistry

Background:

  • Electrokinetic chromatography (EKC) utilizes pseudostationary phases for analyte separation.
  • Polycationic additives can serve as pseudostationary phases in EKC.
  • Understanding analyte interactions with these phases is crucial for method development.

Purpose of the Study:

  • To determine apparent diffusion coefficients (Dapp,i) of neutral analytes in solutions containing poly(diallyidimethylammonium) (PDADMA).
  • To investigate the influence of PDADMA concentration on analyte diffusion.
  • To quantify analyte partitioning between bulk solution and the polymer phase.

Main Methods:

  • Stopped migration method used to measure Dapp,i for six neutral analytes.

Related Experiment Videos

  • Polymeric additive concentrations ranged from 0 to 4% w/w.
  • Analyte partitioning model applied to Dapp,i versus polymer concentration data.

    • Main Results:

    • Apparent diffusion coefficients decreased with increasing PDADMA concentration, up to 40% of the maximum.
    • This decrease was not correlated with a 10-fold increase in solution viscosity.
    • Analyte partitioning constants (ranging from 40 to 170) were derived.

    Conclusions:

    • Analyte diffusion is significantly affected by interactions with the PDADMA pseudostationary phase.
    • The observed changes in Dapp,i reflect analyte partitioning, not bulk viscosity effects.
    • The derived partition constants align with those obtained via traditional EKC methods.