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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Enantiomeric separations by nonaqueous capillary electrophoresis
1Department of Analytical Research, Merck Research Laboratories, Merck & Co., Inc., Rahway, NJ 07065, USA.
Journal of Chromatography. A
|June 6, 2000
Summary
This review covers advances in nonaqueous capillary electrophoresis (NACE) for enantioseparation. It details how organic solvents and parameters like chiral selectors affect enantiomer separation, aiding chiral drug analysis.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Chiral compounds require enantioseparation for pharmaceutical and chemical applications.
- Nonaqueous capillary electrophoresis (NACE) offers an alternative to aqueous methods for separating enantiomers.
Purpose of the Study:
- To review recent advancements in enantioseparation using NACE.
- To discuss the impact of organic solvents on enantiomer mobility, selectivity, and resolution.
- To present applications of NACE for separating racemic mixtures.
Main Methods:
- Review of literature on nonaqueous capillary electrophoresis.
- Analysis of factors influencing enantioseparation in organic media.
- Compilation of reported NACE applications for racemates.
Main Results:
- NACE systems are analogous to aqueous CE but utilize pure organic solvents.
- Organic solvent choice significantly impacts enantiomer mobility, separation selectivity, and resolution.
- Key parameters include chiral selector concentration/type, apparent pH, ionic strength, temperature, and electroosmotic flow control.
Conclusions:
- NACE is a viable technique for enantioseparation, offering unique advantages with organic solvents.
- Understanding solvent effects and optimizing parameters is crucial for effective chiral separations.
- NACE has demonstrated utility in various applications for separating racemic compounds.
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