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Updated: Oct 16, 2026

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
Published on: April 9, 2017
Aspartame degradation study using electrospray ionization mass spectrometry
S Pattanaargson1, C Sanchavanakit
1Department of Chemistry, Faculty of Science, Chulalongkorn University, Payatai, Bangkok 10330, Thailand. psupason@netserv.chula.ac.yh
Abstract:
Electrospray mass spectrometry was used to simultaneously determine aspartame (APM) and five of its degradation products; aspartic acid, aspartylphenylalanine, 5-benzyl-3,6-dioxo-2-piperazieacetic acid (diketopiperazine), phenylalanine, and phenylalanine methyl ester. Under the ionization conditions used, there was no interfering fragmentation for any of the six compounds, i.e., no fragmentation of the compound being tested into other species also being monitored. A study of APM degradation in solution at various pH's and at various temperatures using this method was performed.
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