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Lactic acid buffering by bone and shell in anoxic softshell and painted turtles.

D C Jackson¹, A L Ramsey, J M Paulson

¹Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI 02912, USA. Donald_Jackson@brown. edu.

Physiological and Biochemical Zoology : PBZ

|July 13, 2000

Summary

The softshell turtle

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Area of Science:

Comparative physiology
Biochemistry
Herpetology

Background:

Turtles exhibit varying anoxia tolerance.
Shell mineralization may influence anoxia survival.
Lactate buffering in shells and bones is key.

Purpose of the Study:

Compare anoxia tolerance between Apalone spinifera and Chrysemys picta bellii.
Investigate the role of shell mineralization in anoxia tolerance.
Determine lactate storage in turtle shells and bones during anoxia.

Main Methods:

Measure lactate, ions (Ca2+, Mg2+, Na+, K+), and phosphate in blood, bone, and shell.
Induce anoxia at 10°C for 2 and 9 days in both species.
Analyze shell buffer capacity via incubation at controlled pH.

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Main Results:

Apalone spinifera has a less mineralized shell with higher water content than Chrysemys picta bellii.
Apalone's shell releases significantly less buffer and has lower ion content.
Both species store lactate in shell and bone, but Apalone shows a weaker compensatory response to lactic acidosis.

Conclusions:

Lower shell mineralization and buffer capacity in Apalone correlate with poorer anoxia tolerance.
Shell and bone lactate storage occurs in both species.
Shell properties are critical factors in turtle anoxia survival.