Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transmission Electron Diffraction at 200 eV and Damage Thresholds below the Carbon K Edge.

Stevens1, Chen, Weierstall

  • 1Department of Physics, Arizona State University, Tempe, AZ 85287

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|July 18, 2000
PubMed
Summary

Reducing electron beam energy significantly minimizes molecular damage in organic films, particularly aromatic perylene. This finding opens new avenues for imaging delicate biomolecules using low-energy electrons.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conformation of Fibronectin Fibrils Varies: Discrete Globular Domains of Type III Repeats Detected.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·1999
Same author

A Structural Equation Model of the Effects of Negative Affectivity, Leader-Member Exchange, and Perceived Job Mobility on In-role and Extra-role Performance: A Chinese Case.

Organizational behavior and human decision processes·1999
Same author

Preparation of Palladium Ultrafine Particles in Reverse Micelles.

Journal of colloid and interface science·1999
Same author

New taxoids from the seeds of taxus chinensis

Journal of natural products·1999
Same author

Self-assembly of ordered microporous materials from rod-coil block copolymers

Science (New York, N.Y.)·1999
Same author

Conformational Structure of Triblock Copolymers by FT-Raman and FTIR Spectroscopy.

Journal of colloid and interface science·1999

Area of Science:

  • Materials Science
  • Surface Science
  • Electron Microscopy

Background:

  • Electron beam damage is a critical limitation in analyzing ultra-thin organic films.
  • Understanding molecular damage cross-sections is essential for high-resolution imaging.
  • Low-energy electron transmission (LEET) microscopy offers potential for reduced sample damage.

Purpose of the Study:

  • To investigate the effect of beam energy on molecular damage in aromatic and aliphatic organic films.
  • To correlate electron beam damage with carbon K-shell ionization.
  • To explore the feasibility of imaging biomolecules using low-energy electrons.

Main Methods:

  • Recorded transmission electron diffraction patterns from ultra-thin organic films (aromatic and aliphatic) using a custom low-energy electron transmission (LEET) chamber.

Related Experiment Videos

  • Varied beam energies from 200 eV to 1 keV.
  • Measured molecular damage by observing the fading of diffraction spots and compared with carbon K-shell ionization cross-sections.

    • Main Results:

    • A significant reduction in molecular damage cross-section for perylene films was observed when beam energy decreased from 400 eV to 200 eV.
    • Aliphatic tetracontane showed a smaller threshold energy for damage compared to aromatics.
    • Carbon K-shell ionization strongly correlated with observed damage in aromatic materials above 284 eV.

    Conclusions:

    • Lowering electron beam energy is an effective strategy to minimize damage in organic thin films.
    • The study indicates strong correlation between carbon K-shell ionization and electron beam damage in aromatics.
    • These findings suggest new possibilities for high-resolution imaging of biomolecules with low-energy electrons.