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Physiological state-specific models in estimation of recombinant Escherichia coli fermentation performance.

M Feng1, J Glassey

  • 1Department of Chemical and Process Engineering, University of Newcastle upon Tyne, Newcastle Upon Tyne NE1 7RU, United Kingdom.

Biotechnology and Bioengineering
|July 18, 2000
PubMed
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Chemical fingerprinting, like pyrolysis mass spectrometry, can identify physiological changes in bioprocess cultures. This enables better on-line bioprocess control through state-specific models.

Area of Science:

  • Biotechnology
  • Process Engineering
  • Analytical Chemistry

Background:

  • Advanced bioprocess control is limited by the absence of real-time physiological state measurements.
  • Current methods for estimating key variables often fail to capture dynamic physiological shifts.

Purpose of the Study:

  • To demonstrate the utility of chemical fingerprinting for assessing bioprocess physiological states.
  • To enable the development of physiological state-specific models for enhanced on-line bioprocess control.

Main Methods:

  • Utilized pyrolysis mass spectrometry (PyMS) for chemical fingerprinting of bioprocess cultures.
  • Analyzed spectral data to identify markers of physiological state changes during cultivation.

Main Results:

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  • Pyrolysis mass spectrometry data successfully identified distinct physiological states within the bioprocess.
  • Demonstrated the correlation between chemical fingerprints and the culture's physiological condition.

Conclusions:

  • Chemical fingerprinting provides valuable on-line information on bioprocess physiological states.
  • This approach facilitates the creation of physiological state-specific models for improved on-line control strategies.