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Physiological state-specific models in estimation of recombinant Escherichia coli fermentation performance
1Department of Chemical and Process Engineering, University of Newcastle upon Tyne, Newcastle Upon Tyne NE1 7RU, United Kingdom.
Biotechnology and Bioengineering
|July 18, 2000
Summary
Chemical fingerprinting, like pyrolysis mass spectrometry, can identify physiological changes in bioprocess cultures. This enables better on-line bioprocess control through state-specific models.
Area of Science:
- Biotechnology
- Process Engineering
- Analytical Chemistry
Background:
- Advanced bioprocess control is limited by the absence of real-time physiological state measurements.
- Current methods for estimating key variables often fail to capture dynamic physiological shifts.
Purpose of the Study:
- To demonstrate the utility of chemical fingerprinting for assessing bioprocess physiological states.
- To enable the development of physiological state-specific models for enhanced on-line bioprocess control.
Main Methods:
- Utilized pyrolysis mass spectrometry (PyMS) for chemical fingerprinting of bioprocess cultures.
- Analyzed spectral data to identify markers of physiological state changes during cultivation.
Main Results:
- Pyrolysis mass spectrometry data successfully identified distinct physiological states within the bioprocess.
- Demonstrated the correlation between chemical fingerprints and the culture's physiological condition.
Conclusions:
- Chemical fingerprinting provides valuable on-line information on bioprocess physiological states.
- This approach facilitates the creation of physiological state-specific models for improved on-line control strategies.