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Mass spectrometry in combinatorial chemistry.

C Enjalbal1, J Martinez, J L Aubagnac

  • 1Laboratoire des Aminoacides, Peptides et Protéines-UMR 5810, Universités Montpellier I et II, Montpellier, France.

Mass Spectrometry Reviews
|July 21, 2000
PubMed
Summary

Mass spectrometry is the preferred method for analyzing combinatorial chemistry libraries, enabling efficient identification and quantification of compounds. Automation enhances throughput for large-scale library characterization.

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Area of Science:

  • Chemistry
  • Analytical Chemistry
  • Drug Discovery

Background:

  • Combinatorial chemistry library profiling is crucial for drug discovery.
  • Spectroscopic methods like mass spectrometry, IR, and NMR are used in library synthesis.
  • Efforts focus on assessing library composition, from mixtures to discrete compounds.

Purpose of the Study:

  • To review the application of spectroscopic methods in combinatorial library synthesis and characterization.
  • To highlight the evolution of analytical challenges and solutions in library profiling.
  • To detail methodologies for analyzing both solution-phase and solid-supported compounds.

Main Methods:

  • Mass spectrometry (MS) is highlighted as the method of choice for library analysis.
  • Chromatographic separation coupled with MS provides rapid, sensitive, and informative analyses.

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  • Automated workstations have been developed for high-throughput compound identification and quantification.

    • Main Results:

    • Mass spectrometry offers significant advantages for analyzing complex combinatorial libraries.
    • Increased automation has been essential for efficient identification and quantification of library components.
    • Both solution-based and support-bound methodologies are discussed for comprehensive analysis.

    Conclusions:

    • Mass spectrometry, particularly when coupled with chromatography, is the most effective technique for combinatorial library characterization.
    • Automation is key to achieving high throughput in identifying and quantifying library components.
    • The review covers diverse analytical strategies for diverse library formats in combinatorial chemistry.