Related Experiment Video
Updated: May 11, 2026

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
Published on: August 30, 2013
Thy 1 (theta) antigen on the surface of morphologically distinct brain cell types
Abstract:
Surface cultures of 15-19 day old fetal mouse brain that do not initially express the Thy 1 antigen develop the antigen between 4 and 11 days in culture. The antigen can be detected by immunofluorescence of live cells on the culture plate on two morphologically distinct types of cells. Both neuronal-looking cells and a more general category of cells which includes fibroblastic cells can express the Thy 1 antigen. The antigen is not expressed on certain cells of the glial type. This method enables one to distinguish between different types of brain cells in culture both morphologically and immunologically at the same time.
Related Concept Videos
Enzyme-linked Receptors
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Glial Cells
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

