Cloning and sequencing of sialyltransferase gene from human fetal liver

Based on sequences of the highly conserved segments in the previously cloned sialyltransferases, 150 bp fragments were amplified and sequenced using human fetal liver mRNA as template. One of them (s38) showed 57%-97% identities with the active domains of previously cloned sialyltransferases. Based on the sequence of s38, an oligonucleotide was synthesized and labeled to screen human fetal liver cDNA library. A cDNA encoding alpha 2, 3-sialyltransferase has been isolated. The cDNA sequence included an open reading frame coding for 340 amino acid residues, and the deduced amino acid sequence showed 100% identity with that of human submaxillary gland Gal beta 1, 3GalNAc alpha 2, 3-sialyltransferase, 83.2% identity with that of pig submaxillary gland alpha 2, 3-sialyltransferase. These results suggested that the protein encoded by the cDNA from human fetal liver cDNA library was a Gal beta 1, 3GalNAc alpha 2, 3-sialyltransferase.