Related Experiment Video
Updated: Aug 27, 2026

Single-Molecule Imaging of Nuclear Transport
Published on: June 9, 2010
Characterization of substance P-membrane interaction by transferred nuclear Overhauser effect
1Institut de Pharmacologie et de Biologie Structurale, CNRS, 205 rte de Narbonne, 31077 Toulouse, France.
Abstract:
Substance P, one of the mammalian tachykinins, is known to interact strongly with lipid bilayers and this interaction may play a role in the receptor-peptide recognition process. The conformation of substance P bound to vesicles consisting of perdeuterated phosphatidylcholine has been investigated by means of two-dimensional transferred nuclear Overhauser (trNOE) spectroscopy. Nuclear magnetic resonance data analysis resulted in a unique conformational family characterized by a well-defined conformation of the last seven C-terminal amino acids, which consists of a sequence of nonstandard turns following each other in a helix-like manner. The absence of short- or medium-range trNOE in the N-terminal part indicates its structural flexibility.
More Related Videos
Related Concept Videos
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
Atomic Nuclei: Magnetic Resonance
NMR Spectroscopy: Spin–Spin Coupling
Nuclear Overhauser Enhancement (NOE)
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)
Double Resonance Techniques: Overview
Spin decoupling is usually achieved by...

