[Fluocinonide Reference Standard (Control 981) of National Institute of Health Sciences]

The raw material of fluocinonide was examined for preparation of the "Fluocinonide Reference Standard (Control 981)". The analytical data obtained were: UV spectrum, lambda max of 237.4 nm; IR spectrum, same as that of the Fluocinonide Reference Standard (Control 841); optical rotation, [alpha]20(D) = + 84.4 degree, thin-layer chromatography, one impurity detected; high-performance liquid chromatography, three impurities detected and total amount estimated to be about 0.20%; loss on drying, 0.15%; assay by HPLC, 99.6% Based on the above results, the raw material was authorized as the Fluocinonide Reference Standard (Control 981) of the National Institute of Health Sciences.