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Related Experiment Videos

Penetration enhancers assessed by corneoxenometry.

V Goffin1, F Henry, C Piérard-Franchimont

  • 1Belgian SSTC Research Center 5596, Department of Dermatopathology, University Medical Center of Liège, Belgium.

Skin Pharmacology and Applied Skin Physiology
|August 15, 2000
PubMed
Summary
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Corneoxenometry, a new bioassay, effectively measures chemical enhancer effects on skin barrier function. This method offers a rapid, cost-effective alternative to animal testing for assessing skin penetration and irritation potential.

Area of Science:

  • Dermatology and Cosmetology
  • Biophysical Chemistry
  • Toxicology

Background:

  • Chemical enhancers significantly impact the stratum corneum's barrier function through various mechanisms.
  • Determining the synergistic or antagonistic effects of these enhancers in mixtures presents a significant challenge.
  • Existing methods for assessing skin barrier alteration are often time-consuming and rely on animal testing.

Purpose of the Study:

  • To evaluate the comparative effects of chemical enhancers, specifically ethanol and laurocapram, on skin barrier function.
  • To assess the impact of a multi-component formulation containing N-acetyl-L-cysteine, urea, and salicylic acid on skin barrier properties.
  • To validate Corneoxenometry as a reliable and rapid bioassay for predicting skin barrier alterations.

Main Methods:

Related Experiment Videos

  • Corneoxenometry, a bioassay utilizing reflectance colorimetry, was employed to assess xenobiotic interactions with corneocytes.
  • Comparative analysis of ethanol and laurocapram at various dilutions was conducted.
  • A propylene-glycol-based gel formulation with varying concentrations of N-acetyl-L-cysteine, urea, and salicylic acid was tested.

Main Results:

  • The Relative Index of Irritancy (RII) was calculated for each tested formulation.
  • RII serves as a surrogate marker for lipid removal, stratum corneum disorganization, and protein denaturation.
  • The study demonstrated Corneoxenometry's capability to quantify the effects of different chemical enhancers and formulations.

Conclusions:

  • Corneoxenometry is a viable, cost-effective, and rapid alternative to traditional animal testing for evaluating skin barrier function.
  • The bioassay provides a good prediction of barrier function alterations caused by chemical enhancers.
  • This method aids in understanding the complex interactions of penetration enhancers and active ingredients in cosmetic and pharmaceutical formulations.