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[Use of cryopreserved sperm in the microagglutination test]
P Seifertová1, Z Ulcová-Gallová, P Panzner
1Gynekologicko-porodnická klinika LF UK a FN v Plzni.
Objective:
The aim of this study was to find out the behavior of sperm cryopreserved in microagglutination test (TAT).
Methods:
TAT using native sperm pertains to the screening methods in spermagglutinating antibodies. Spermatozoa cryopreserved in egg-yolk medium, just in seminal plasma or in the commercial made medium--Medi-Cult (Denmark) were used in this study.
Setting:
Department of Gynaecology and Obstetrics, Institute of Clinical Immunology and Allergology Medical Faculty of Charles University, Pilsen.
Results:
367 tests were performed together. It includes 108 examinations with native spermatozoa, 59 with spermcryopreserved in egg-yolk medium, 109 with spermcryopreserved just in seminal plasma and 91 in Medi-Cult medium. The correspondance of results with native and cryopreserved sperm was found in 24% for egg-yolk medium, in 69% for seminal plasma and in 77% for commercial medium Medi-Cult.
Conclusion:
The results show that spermcryopreservation in Medi-Cult medium or just in the seminal plasma are able to be used in TAT for detection of spermagglutinating antibodies.
Insights
Sperm cryopreservation in seminal plasma or Medi-Cult medium is effective for the microagglutination test (TAT) to detect spermagglutinating antibodies. Egg-yolk medium showed lower correspondence with native sperm results.
Area of Science:
- Reproductive immunology
- Spermatozoa cryobiology
Context:
- The microagglutination test (TAT) is a screening method for detecting spermagglutinating antibodies.
- Evaluating the efficacy of cryopreserved sperm in diagnostic assays is crucial for reproductive health assessments.
Purpose:
- To assess the behavior and diagnostic utility of cryopreserved sperm in the microagglutination test (TAT).
- To compare the effectiveness of different sperm cryopreservation media (egg-yolk, seminal plasma, Medi-Cult) for TAT.
Summary:
- A total of 367 TAT were performed using native and cryopreserved spermatozoa.
- Cryopreservation in Medi-Cult medium (77% correspondence) and seminal plasma (69% correspondence) yielded results comparable to native sperm.
- Sperm cryopreserved in egg-yolk medium showed lower correspondence (24%) with native sperm results.
Impact:
- Cryopreservation of sperm in seminal plasma or Medi-Cult medium is a viable option for TAT, enhancing its application in diagnosing immunological infertility.
- These findings support the use of cryopreserved sperm in immunological testing, offering flexibility and potentially reducing costs associated with fresh sample preparation.